Test Overview

A rheumatoid factor (RF) blood test measures the amount of the RF antibody present in the blood. Normally, antibodies are produced by the immune system to help destroy and get rid of invading bacteria and viruses that can cause disease. But the RF antibody can attach to normal body tissue and cause damage.

A high level of RF can be caused by several autoimmune diseases (including rheumatoid arthritis) and some infections. Sometimes a healthy person can have a high RF level.

The amount of RF in blood can be measured in two ways:

Agglutination tests.

One test method mixes blood with tiny rubber (latex) beads that are covered with human antibodies. If RF is present, the latex beads clump together (agglutinate). This method is best used as a first-time screening test for rheumatoid arthritis. Another agglutination test mixes the blood being tested with a sheep's red blood cells that have been covered with rabbit antibodies. If RF is present, the red blood cells clump together. This method is often used to confirm that RF is present.

Nephelometry test.

This test mixes the blood being tested with antibodies that cause the blood to clump if RF is present. A laser light is shined on the tube containing the mixture. Then the amount of light blocked by the blood sample is measured. As levels of RF increase, more clumping occurs. This causes a cloudier sample and allows less light to pass through the tube.